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HelpTest ID: THSD7 Thrombospondin Type-1 Domain-Containing 7A Antibodies, Serum
Specimen Required
Supplies: Sarstedt 5 mL Aliquot Tube (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container: Plastic vial
Specimen Volume: 1 mL
Collection Information: Centrifuge and aliquot serum into plastic vial within 2 hours of collection.
Useful For
Distinguishing primary from secondary membranous nephropathy cases with antibodies against THSD7A
Method Name
Indirect Immunofluorescence Assay (IFA)
Reporting Name
THSD7A Ab, SSpecimen Type
SerumSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 14 days | ||
| Ambient | 8 hours |
Clinical Information
Recently, autoantibodies against phospholipase A2 receptor (PLA2R) in the kidney were determined to be the major target antigen for patients with idiopathic/primary membranous nephropathy (MN).(1) Approximately 70% of patients with primary MN circulate anti-PLA2R antibodies, and in the remaining 30% (who are PLA2R-negative), anti-thrombospondin type-1 domain-containing 7A (THSD7A) was shown to have approximately a 10% prevalence (or about 3% of all primary MN patients).(2) Mouse podocytes express THSD7A and introduction of anti-THSD7A autoantibodies induces MN in murine models. Mouse podocytes do not express PLA2R so exogenous administration of anti-PLA2R does not recapitulate membranous nephropathy in mice.(3) Additionally, THSD7A has been described as a potential tumor antigen and, thus, it has been suggested that THSD7A-positive patients merit a thorough cancer screening.(4)
Reference Values
Negative
Interpretation
Therapy outcome can be monitored by measuring the antibody titer. A titer increase, decrease, or disappearance generally precedes a change in clinical status. Thus, the determination of the antibody titer has a high predictive value with respect to clinical remission, relapse, or risk assessment after kidney transplantation.
Clinical Reference
1. Beck LH Jr, Bonegio RG, Lambeau G, et al: M-type phospholipase A2 receptor as target antigen in idiopathic membranous nephropathy. N Engl J Med. 2009;361:11-21. doi: 10.1056/NEJMoa0810457
2. Tomas NM, Beck LH Jr, Meyer-Schwesinger C, et al: Thrombospondin type-1 domain-containing 7A in idiopathic membranous nephropathy. N Engl J Med. 2014;371:2277-2287.doi: 10.1056/NEJMoa1409354
3. Tomas NM, Hoxha E, Reinicke AT, et al: Autoantibodies against thrombospondin type 1 domain-containing 7A induce membranous nephropathy. J Clin Invest. 2016;126(7):2519-2532. doi: 10.1172/JCI85265
4. Stahl PR, Hoxha E, Wiech T, Schroder C, Simon R, Stahl RA: THSD7A expression in human cancer. Genes Chromosomes Cancer. 2017;56:314-327. doi: 10.1002/gcc.22440
Day(s) Performed
Tuesday
Report Available
3 to 7 daysTest Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
86255
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| THSD7 | THSD7A Ab, S | 93339-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| THSD7 | THSD7A Ab, S | 93339-0 |
Forms
If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.
mcl-renalai, mcl-gdnephrotic