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Test ID: ALPRT Alport (Collagen IV Alpha 5 and Alpha 2) Immunofluorescent Stain, Renal Biopsy


Shipping Instructions


1. Advise shipping frozen specimens (unstained slides or tissue block) in Styrofoam transportation coolers filled with dry ice to ensure specimens are received at required specimen stability temperature.

2. Attach the green "Attention Pathology" address label (T498) to the outside of the transport container before putting into the courier mailer.



Necessary Information


A pathology/diagnostic report is required.



Specimen Required


Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Unstained slides (unfixed)

Source: Kidney tissue or skin tissue

Slides: 1 Slide

Collection Instructions: Submit 1 frozen tissue unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections should be 4-microns thick, centered on the slide, and shipped on dry ice.

 

Acceptable:

Specimen Type: Unfixed tissue block (O.C.T)

Source: Kidney tissue or skin tissue

Specimen Volume: Entire specimen

Collection Instructions:

1. Embed in O.C.T. compound.

2. Freeze specimen and ship on dry ice.

 

Acceptable:

Specimen Type: Wet tissue

Source: Kidney tissue or skin tissue

Supplies: Renal Biopsy Kit (T231)

Container/Tube: Renal Biopsy Kit, Zeus/Michel's

Specimen Volume: Entire specimen

Collection Instructions:

1. Collect specimens according to the instructions in Renal Biopsy Procedure for Handling Tissue for Light Microscopy, Immunofluorescent Histology, and Electron Microscopy.

2. If standard immunoglobulin and complement immunofluorescence has already been performed, ship the residual frozen tissue (must contain glomeruli) on dry ice.


Forms

1. Renal Biopsy Patient Information

2. If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.

Useful For

Assisting in the diagnosis of hereditary nephritis (Alport syndrome)

Method Name

Direct Immunofluorescence

Reporting Name

ALPORT Immunofluorescence

Specimen Type

Special

Specimen Stability Information

Specimen Type Temperature Time Special Container
Special Frozen

Clinical Information

Alport syndrome is a hereditary disease of basement membrane collagen type IV. Variants in collagen IV alpha genes cause characteristic abnormal immunofluorescence staining patterns within the glomerular basement membrane. Alport syndrome is characterized by hematuria, proteinuria, progressive kidney failure, and high-tone sensorineural hearing loss.

Reference Values

An interpretive report will be provided.

Interpretation

This test (when not accompanied by a pathology consultation request) will be reported as one of the following:

1) Normal pattern

2) Consistent with X-linked hereditary nephritis

3) Consistent with autosomal hereditary nephritis

 

If additional interpretation or analysis is needed, request PATHC / Pathology Consultation along with this test and send the corresponding renal pathology light microscopy and immunofluorescence (IF) slides (or IF images on a CD), electron microscopy images (prints or CD), and the pathology report.

Clinical Reference

1. Kagawa M, Kishiro Y, Naito I, et al. Epitope-defined monoclonal antibodies against type-IV collagen for diagnosis of Alport's syndrome. Nephrol Dial Transplant. 1997;12(6):1238-1241

2. Hashimura Y, Nozu K, Kaito H, et al. Milder clinical aspects of X-linked Alport syndrome in men positive for the collagen IV alpha 5 chain. Kidney Int. 2014;85(5):1208-1213

3. Kamiyoshi N, Nozu K, Fu XJ, et al. Genetic, clinical, and pathologic backgrounds of patients with autosomal dominant Alport syndrome. Clin J Am Soc Nephrol. 2016;11(8):1441-1449

4. Said SM, Fidler ME, Valeri AM, et al. Negative staining for COL4A5 correlates with worse prognosis and more severe ultrastructural alterations in males with Alport syndrome. Kidney Int Rep. 2016;2(1):44-52

Day(s) Performed

Monday through Friday

Report Available

1 to 2 days

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

88346

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ALPRT ALPORT Immunofluorescence In Process

 

Result ID Test Result Name Result LOINC Value
71285 Interpretation 50595-8
71268 Participated in the Interpretation No LOINC Needed
71269 Report electronically signed by 19139-5
71270 Addendum 35265-8
71271 Gross Description 22634-0
71272 Material Received 81178-6
71619 Disclaimer 62364-5
71848 Case Number 80398-1

Specimen Minimum Volume

See Specimen Required

Mayo Clinic Laboratories | Renal Diagnostics Catalog Additional Information:
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